Journal: iScience

Article Title: Temporal and spatial dynamics of immune cells in spontaneous liver transplant tolerance

doi: 10.1016/j.isci.2023.107691

Figure Lengend Snippet: List of metal-labeled antibodies used for mass cytometry

Article Snippet: Anti-CD223 (LAG-3) , Fluidigm , 3174019C.

Techniques:

Journal: iScience

Article Title: Temporal and spatial dynamics of immune cells in spontaneous liver transplant tolerance

doi: 10.1016/j.isci.2023.107691

Figure Lengend Snippet:

Article Snippet: Anti-CD223 (LAG-3) , Fluidigm , 3174019C.

Techniques: Transplantation Assay, Recombinant, Staining, Cell Isolation, Software, Cytometry, Flow Cytometry

Journal: Molecular Therapy Oncology

Article Title: Minute virus of mice shows oncolytic activity against pancreatic cancer cells exhibiting a mesenchymal phenotype

doi: 10.1016/j.omton.2024.200780

Figure Lengend Snippet: Antibodies for flow cytometry used during this study

Article Snippet: LAG-3 , VioBright FITC , C9B7W , Miltenyi , 130-120-170.

Techniques: Flow Cytometry

Journal: Cell Reports Medicine

Article Title: Ce6 derivative photodynamic therapy triggers PANoptosis and enhances antitumor immunity with LAG3 blockade in cutaneous squamous cell carcinoma

doi: 10.1016/j.xcrm.2025.102239

Figure Lengend Snippet: Combination of STBF-PDT, a STING agonist, and LAG3 blockade induces abscopal effects in vivo (A) Tumor growth curves for different treatment groups in unilateral mice model. n ≥ 3 mice/group. (B) Growth curves of primary and distant tumors across different treatment groups in bilateral mice model. n = 5 mice/group. (C–F) LAG3, PD-1, TIM3, and TIGIT with their ligand expression in cSCC cells and murine tumors under various treatment conditions. Quantification based on at least three biological replicates ( n ≥ 3). (G) Western blot analysis showing the expression of LAG3 ligands and PD-1 in cSCC cells following different treatment. (H) Representative images illustrating the expression and localization of Lgals3 in primary and distant tumors across different treatment groups in the bilateral mouse model. Scale bars: 50 μm. Quantitative analysis based on three biological replicates ( n = 3). (I and J) LAG3 expression analysis in across various squamous cell carcinoma types using Gene Expression Omnibus and The Cancer Genome Atlas Program database. (K) mRNA analysis of LAG3 transcription levels in patients with cSCC. n = 6 biologically independent samples/group. (L) LAG3 transcription levels in tumor versus paracancerous tissues. Data pooled from independent experiments ( n = 9 biologically independent samples). (M) Schematic of treatment regimen in bilateral tumor-bearing mice; representative images of cSCC-transplanted mice from each treatment group. (N) Growth curves of primary and distant tumors across different treatment groups. n ≥ 5 mice/group. (O) Representative H&E-stained images of primary tumors. (P) Representative Ki67 immunohistochemistry images of primary tumors. Data are represented as mean ± SEM. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Article Snippet: Mice in the polytherapy group also received anti-LAG3 monoclonal antibody (200μg; BioXCell, BP0174) via intraperitoneal injection at seven-day intervals.

Techniques: In Vivo, Expressing, Western Blot, Gene Expression, Staining, Immunohistochemistry

Journal: Cell Reports Medicine

Article Title: Ce6 derivative photodynamic therapy triggers PANoptosis and enhances antitumor immunity with LAG3 blockade in cutaneous squamous cell carcinoma

doi: 10.1016/j.xcrm.2025.102239

Figure Lengend Snippet: Polytherapy modulates MDSC activity, enhancing T cell expansion and activation in vitro (A) Schematic and representative images of transwell chemotaxis assays showing CFSE + MDSC migration toward XL-50 cells under various treatments. Quantification plots representing one of three biological replicates; n = 5 fields/group. (B) ROS detection in MDSCs co-cultured with XL-50 cells. Quantification plots representing one of three biological replicates; n = 4 fields/group. (C) NO release measurements by MDSCs based on three biological replicates ( n = 3). (D) Arginase activity in MDSCs under different conditions based on three biological replicates ( n = 3). (E) Expression of arginase1, iNOS, Idol1, Hif1a, Tgfb2, and Adam17 in MDSCs under different treatments based on three biological replicates ( n = 3). (F) Schematic and representative images of T cell chemotaxis cocultured with MDSCs (T cells: MDSCs = 1:1) and XL-50 cells under indicated treatments. Quantification plots representing one of three biological replicates; n = 5 fields/group. (G) Quantification of CFSE + T cell proportions (vehicle, CFSE − T cell: CFSE + T cell = 1:1; control, anti-LAG3, STBF-PDT + ADU-S100; polytherapy, CFSE − MDSCs: CFSE + T cell = 1:1) based on three biological replicates ( n = 3). (H) Expression of Ox40, 4-1BB, granzyme B, and perforin in T cells without CD3/CD28 stimulation based on three biological replicates ( n = 3). (I) ELISA quantification of chemokines (Ccl2, Cxcl1, Cxcl12, and Cxcl8) in XL-50 supernatants. n = 6 biological replicates. Data are represented as mean ± SEM. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Article Snippet: Mice in the polytherapy group also received anti-LAG3 monoclonal antibody (200μg; BioXCell, BP0174) via intraperitoneal injection at seven-day intervals.

Techniques: Activity Assay, Activation Assay, In Vitro, Chemotaxis Assay, Migration, Cell Culture, Expressing, Control, Enzyme-linked Immunosorbent Assay

Journal: Cell Reports Medicine

Article Title: Ce6 derivative photodynamic therapy triggers PANoptosis and enhances antitumor immunity with LAG3 blockade in cutaneous squamous cell carcinoma

doi: 10.1016/j.xcrm.2025.102239

Figure Lengend Snippet: Polytherapy provides tumor suppression in the UV-irradiated cSCC mice model (A) Schematic representation of UV-irradiated mice treated with STBF-PDT and ADU-S100, with anti-LAG3 antibody. (B) Representative images of UV-induced cSCC mice from each treatment group, taken every 6 days. (C) Tumor growth curves and tumor counts for different treatment groups. n = 6 mice/control group, n = 4 mice/anti-LAG3 group, n = 4 mice/STBF-PDT + ADU-S100 group, n = 6 mice/polytherapy group. (D) ELISA quantification of Ccl2, Ccl7, Csf1, and Csf2 in murine tumor supernatants ( n ≥ 4 mice/group). (E) Representative immunohistochemical staining of CD8 + T cells and Gr1 + cells (MDSCs) in tissue sections. Quantification plots based on 9 fields per group ( n = 9) from at least three biologically independent mice. (F) Representative flow cytometry plots of dendritic cells in inguinal lymph nodes of cSCC mice. Plots showing the quantification of CD11c + /MHCII + /CD11b − DC1s and CD11c + /MHCII + /CD11b + DC2s in inguinal lymph nodes ( n ≥ 4 mice/group). (G) Immunofluorescence imaging of cDC2(CD11c + /MHCII + /CD11b + ) in tumors. Quantification plots based on 9 fields ( n = 9) from at least three biologically independent mice. (H) Multiplex cytokine analysis of MDSCs and Treg chemotaxis-associated genes in mice serum. n = 4 mice/control group, n = 5 mice/polytherapy group. (I) UMAP plot representing cell distribution, color-coded by annotated cell types (left), alongside an overview of spatial transcriptomic sections (right). H&E staining identifying tumor invasive regions (gray points). (J) GO chord analysis illustrating the top upregulated and downregulated genes post polytherapy compared to controls. (K) GO enrichment analysis performed on tumor invasive regions, comparing two treatment groups. (L) Comparative CellChat analysis highlighting interaction numbers and strengths across clusters between control and polytherapy groups. (M) ST data illustrating infiltration of effector T cells and memory T cell subsets. (N) Spatial feature plots of marker genes between the two groups. Data are represented as mean ± SEM. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Article Snippet: Mice in the polytherapy group also received anti-LAG3 monoclonal antibody (200μg; BioXCell, BP0174) via intraperitoneal injection at seven-day intervals.

Techniques: Irradiation, Control, Enzyme-linked Immunosorbent Assay, Immunohistochemical staining, Staining, Flow Cytometry, Immunofluorescence, Imaging, Multiplex Assay, Chemotaxis Assay, Marker

Journal: Cell Reports Medicine

Article Title: High-dimensional profiling of pediatric immune responses to solid organ transplantation

doi: 10.1016/j.xcrm.2023.101147

Figure Lengend Snippet: Antibodies used for CyTOF staining

Article Snippet: anti-human LAG-3-150Nd (11C3C65) , Standard Biotools , Cat. #3150030B.

Techniques:

Journal: Cell Reports Medicine

Article Title: High-dimensional profiling of pediatric immune responses to solid organ transplantation

doi: 10.1016/j.xcrm.2023.101147

Figure Lengend Snippet:

Article Snippet: anti-human LAG-3-150Nd (11C3C65) , Standard Biotools , Cat. #3150030B.

Techniques: Recombinant, Antibody Labeling, Staining, Blocking Assay, Software